| Titre : | ASSESSMENT OF WINTER WHEAT PERFORMANCE UNDER DIFFERENT AMMONIUM NUTRITION REGIMES AND INVESTIGATION OF A CANDIDATE GENE ASSOCIATED WITH AMMONIUM USE EFFICIENCY |
| Auteurs : | MHAMDI Oumayma, Auteur ; GHAOUTI Lamiae, Auteur |
| Type de document : | texte imprimé |
| Editeur : | Rabat : IAV Hassan II, 2023 |
| Format : | 42 |
| Langues: | Français |
| Mots-clés: | Nitrogen Use Efficiency ; Genome-wide association study ; Wheat |
| Résumé : |
Although 3.5 billion people relied on nitrogen fertilisers in 2015, the environmental damage caused by nitrate fertilisers has forced the world to improve Nitrogen Use Efficiency (NUE), defined as grain production per unit of available nitrogen in the soil to preserve the environment from pollution. Ammonium use efficiency could be a potential solution to improve NUE. Therefore, a field trial and a hydroponic experiment were conducted to study the performance of eight varieties under three nitrogen nutrition modalities with a total amount of 180 kg/ha each. The first treatment is ammonium sulfate, stabilised with 0.02% 3,4-dimethyl pyrazole phosphate (DMPP) supplied in four fractions (AS+DMPP-4). The second treatment is Ammonium Nitrate 27% supplied in four fractions (AN-4). The third regime is 27% ammonium nitrate used in three fractions without any application at sowing. The results of the field trials showed that the performance of the varieties according to above-ground biomass dry weight, relative chlorophyll content, and canopy coverage of the eight varieties diverged.
The Tuckey test was used to investigate the response and preference of each variety to the three fertiliser regimes. In terms of aboveground biomass dry weight, all varieties preferred AS+DMPP-4 except Graham and Julius. Cellule and Julius prefer AS+DMPP-4 for relative chlorophyll content during the tillering growth stage (T2). At the stem elongation stage (T3), Boisseau and KWSSmart showed a preference for AN. The varieties had no preference for any treatment except Attlass, Pajero, and Boisseau, which preferred AN for canopy establishment In parallel, using the results of a genome-wide association study (GWAS), a potential gene previously identified as significantly associated with ammonium uptake was used to find its homologs in Arabidopsis thaliana. T-DNA insertion lines were multiplied to determine homozygous lines by PCR amplification using gene-specific primers. The results showed that GK-713E11.14 from Gabi-kat is a homozygous line. |
| En ligne : | http://10.2.0.27//cda/ebooks/MHAMDI_Oumayma_2023.pdf.pdf |
Exemplaires (1)
| Code-barres | Cote | Support | Localisation | Section | Disponibilité |
|---|---|---|---|---|---|
| 200041053 | 10155 | Support papier | Salle des thèses/PFE (RDC) | Ingénieur Agronome | Consultation sur place Exclu du prêt |
